You may not be able to see this in the Debug Mode. You can check if the number of collision checks suddenly spikes, or lots of instances are created for some object. Maybe you can see something on the CPU/GPU Profiler tab.
Does the game freeze permanently or for a moment? If you've narrowed it down to specific events, try disabling sub-events and actions..
I can't imaging how adding new layout can cause any issues with the Destroy action.