shinkan, very strange. To add to this, I just played around with it a bit more using Squids example but I added another layer to it.
If you play this from the event window it should show the blank newly created layout 3.
What I noticed was that if I keep it on the event window and put layout 1 on the bottom in the project bar - then hit play - It will still open up to the blank screen of layout 3 (using the on screen play button).
But if I put layout 1 at the bottom of the project bar and then click on any layout tab and back to the event sheet it will play whatever is on the bottom (in this case layout 1)
Maybe this is why we are reporting different things? If not I have no idea! :)
Operating system & service pack:
Windows 7 64bit