It's much easier to help if you share a project file.
If I had to guess, try changing to an integer option for the fullscreen mode, e.g. letterbox integer scale. Otherwise it uses fractional scales, and that doesn't work nicely with pixel art (that's just a fact of computer graphics and will affect any software you use, including Unity). Then make sure you are using 'Nearest' sampling, and there shouldn't be anything blurry.
You can also create a new project and check the Optimize for pixel art option, which sets all the appropriate options for you automatically, so you don't have to try to figure out the right combination yourself.