When you add the action 'On Start Of Layout' you select the first layout.
If you mean that you're going to be restarting the tutorial layout a second time and you don't want the object to appear again, set up a global variable 'TutorialFirstRun' and set to 1.
Add Action > System > On Start Of Layout
- (If TutorialFirstRun=1) Add Event > System > Create Object
- Set 'TutorialFirstRun' to 0
When the layout is restarted, global variable should remain at 0 and stop the object creation